Intermediate

Part:BBa_K1904005:Design

Designed by: Enrique Chimal Juarez, Roger Milton Rubio S�nchez, Enrique Arturo Salas Palomino, Brian Morteo Luna   Group: iGEM16_TecCEM   (2016-06-07)


Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was optimised for its usage in RFC 25 standard and in E. coli as chassis. It is designed as a Freiburg N-part and it does not contain restriction site for NgoMIV.


Source

Acinetobacter baumannii phage Abp1 and bacteriophage T4

References